Journal: Materials

Article Title: Surface Modifications of Titanium Aluminium Vanadium Improve Biocompatibility and Osteogenic Differentiation Potential

doi: 10.3390/ma14061574

Figure Lengend Snippet: The influence of TiAl 6 V 4 surface modifications on the expression of adhesion molecules. ( a ) Fibronectin expression showed no changes between the groups. The relative gene expression of ( b ) vinculin, and ( c ) the integrin subunits ITG-β1, and ( d – f ) ITG-α1, -α3, α5 was presented (mean ± SD; n = 8; measured in triplicates). Statistical significances are defined as follows: *** p < 0.001; ** p < 0.01; * p < 0.05.

Article Snippet: After 4 d incubation on the different TiAl 6 V 4 material discs, undiluted cell culture supernatants were analysed using the human Fibronectin ELISA kit (Abcam, Cambridge, UK) according to the corresponding protocol.

Techniques: Expressing

Journal: Tissue Engineering Part A

Article Title: A Strategy to Enhance Secretion of Extracellular Matrix Components by Stem Cells: Relevance to Tissue Engineering

doi: 10.1089/ten.tea.2017.0060

Figure Lengend Snippet: Figure 7: Immunostaining of Fibronectin and Collagen. Immunofluorescence staining of

Article Snippet: The RIPA solubilized samples were diluted at 1:40 dilution with ELISA sample diluent, followed by fibronectin ELISA analysis (Human Fibronectin ELISA Kit, Bosterbio) according to the manufacturer’s protocol.

Techniques: Immunostaining, Immunofluorescence, Staining

Journal: Tissue Engineering Part A

Article Title: A Strategy to Enhance Secretion of Extracellular Matrix Components by Stem Cells: Relevance to Tissue Engineering

doi: 10.1089/ten.tea.2017.0060

Figure Lengend Snippet: Figure 9: Concentration dependent stimulation of fibronectin by hADSCs. A) KTTKS (P0),

Article Snippet: The RIPA solubilized samples were diluted at 1:40 dilution with ELISA sample diluent, followed by fibronectin ELISA analysis (Human Fibronectin ELISA Kit, Bosterbio) according to the manufacturer’s protocol.

Techniques: Concentration Assay

Journal: Drug Delivery

Article Title: Mimicking chronic glaucoma over 6 months with a single intracameral injection of dexamethasone/fibronectin-loaded PLGA microspheres

doi: 10.1080/10717544.2022.2096712

Figure Lengend Snippet: Elaboration scheme of dexamethasone-fibronectin co-loaded PLGA microspheres. Created with BioRender.com.

Article Snippet: Fibronectin quantification was performed by ELISA technique using a Fibronectin ELISA Kit (DuoSet® Human Fibronectin DY1918-05, R&D Systems, Minneapolis, MN, USA).

Techniques:

Journal: Drug Delivery

Article Title: Mimicking chronic glaucoma over 6 months with a single intracameral injection of dexamethasone/fibronectin-loaded PLGA microspheres

doi: 10.1080/10717544.2022.2096712

Figure Lengend Snippet: Dexamethasone-fibronectin co-loaded PLGA microspheres morphology. Scanning (left and center) and transmission (right) electron microscopy images.

Article Snippet: Fibronectin quantification was performed by ELISA technique using a Fibronectin ELISA Kit (DuoSet® Human Fibronectin DY1918-05, R&D Systems, Minneapolis, MN, USA).

Techniques: Transmission Assay, Electron Microscopy

Journal: Drug Delivery

Article Title: Mimicking chronic glaucoma over 6 months with a single intracameral injection of dexamethasone/fibronectin-loaded PLGA microspheres

doi: 10.1080/10717544.2022.2096712

Figure Lengend Snippet: Cumulative in vitro release of fibronectin from dexamethasone-fibronectin co-loaded PLGA microspheres.

Article Snippet: Fibronectin quantification was performed by ELISA technique using a Fibronectin ELISA Kit (DuoSet® Human Fibronectin DY1918-05, R&D Systems, Minneapolis, MN, USA).

Techniques: In Vitro

Journal: Drug Delivery

Article Title: Mimicking chronic glaucoma over 6 months with a single intracameral injection of dexamethasone/fibronectin-loaded PLGA microspheres

doi: 10.1080/10717544.2022.2096712

Figure Lengend Snippet: Cumulative in vitro release of dexamethasone from dexamethasone-fibronectin co-loaded PLGA microspheres.

Article Snippet: Fibronectin quantification was performed by ELISA technique using a Fibronectin ELISA Kit (DuoSet® Human Fibronectin DY1918-05, R&D Systems, Minneapolis, MN, USA).

Techniques: In Vitro

Journal: Drug Delivery

Article Title: Mimicking chronic glaucoma over 6 months with a single intracameral injection of dexamethasone/fibronectin-loaded PLGA microspheres

doi: 10.1080/10717544.2022.2096712

Figure Lengend Snippet: Intraocular pressure in the dexamethasone-fibronectin co-loaded microsphere (MsDexaFibro) model. a: Intraocular pressure curve over 6 months. b: Percentage of ocular hypertensive eyes (>20 mmHg). c: Percentage of corticosteroid response and tendency in right eyes over the study. d: Averaged percentage of corticosteroid response in right eyes. Low: <6 mmHg increase; medium: 6–15 mmHg increase; high: >15 mmHg increase. Abbreviations: IOP: intraocular pressure; RE: right eye; LE: left eye; w: week; OHT: ocular hypertension; %: percentage *: p < 0.05; #: p < 0.02 (Bonferroni Correction for multiple comparisons).

Article Snippet: Fibronectin quantification was performed by ELISA technique using a Fibronectin ELISA Kit (DuoSet® Human Fibronectin DY1918-05, R&D Systems, Minneapolis, MN, USA).

Techniques:

Journal: Drug Delivery

Article Title: Mimicking chronic glaucoma over 6 months with a single intracameral injection of dexamethasone/fibronectin-loaded PLGA microspheres

doi: 10.1080/10717544.2022.2096712

Figure Lengend Snippet: Neuroretina functionality, measured by dark- and light-adapted electroretinography (ERG), in the dexamethasone-fibronectin co-loaded microspheres (MsDexaFibro) model over 6 months of follow-up. MsDexafibro: microspheres loaded with dexamethasone and fibronectin; RE: right eye; LE: left eye; w: week; DA: dark-adapted; LA: light-adapted; μV: microvolts; ms: milliseconds.

Article Snippet: Fibronectin quantification was performed by ELISA technique using a Fibronectin ELISA Kit (DuoSet® Human Fibronectin DY1918-05, R&D Systems, Minneapolis, MN, USA).

Techniques:

Journal: Drug Delivery

Article Title: Mimicking chronic glaucoma over 6 months with a single intracameral injection of dexamethasone/fibronectin-loaded PLGA microspheres

doi: 10.1080/10717544.2022.2096712

Figure Lengend Snippet: A Neuro-retinal thickness in microns by OCT throughout 6-month follow-up. B. Average thickness percentage loss by OCT in microspheres co-loaded with dexamethasone and fibronectin (MsDexaFibro) model up to 6-month follow-up. MsDexaFibro: microspheres co-loaded with dexamethasone and fibronectin; RE: right eye; LE: left eye; OCT: optical coherence tomography; RNFL: Retinal Nerve Fiber Layer; GCL: Ganglion Cell Layer complex; average thickness in microns (μm); w: week; %: percentage.

Article Snippet: Fibronectin quantification was performed by ELISA technique using a Fibronectin ELISA Kit (DuoSet® Human Fibronectin DY1918-05, R&D Systems, Minneapolis, MN, USA).

Techniques: Tomography

Journal: Drug Delivery

Article Title: Mimicking chronic glaucoma over 6 months with a single intracameral injection of dexamethasone/fibronectin-loaded PLGA microspheres

doi: 10.1080/10717544.2022.2096712

Figure Lengend Snippet: Neuro-retinal loss rate measured by optical coherence tomography (OCT) in the co-loaded with dexamethasone and fibronectin microspheres (MsDexaFibro) model. MsDexaFibro: microspheres co-loaded with dexamethasone and fibronectin; RE: right eye; LE: left eye; w: week; RNFL: Retinal Nerve Fiber Layer; GCL: Ganglion Cell Layer complex.

Article Snippet: Fibronectin quantification was performed by ELISA technique using a Fibronectin ELISA Kit (DuoSet® Human Fibronectin DY1918-05, R&D Systems, Minneapolis, MN, USA).

Techniques: Tomography

Journal: Drug Delivery

Article Title: Mimicking chronic glaucoma over 6 months with a single intracameral injection of dexamethasone/fibronectin-loaded PLGA microspheres

doi: 10.1080/10717544.2022.2096712

Figure Lengend Snippet: Ganglion cell analysis in glaucomatous eyes. A. Ganglion cells were counted in four areas (squares) of a radial section of the retina passing through the optic nerve. B. Two representative images of the retina marked with anti-Brn3a corresponding to a left non-intervened eye (LE) and a right eye (RE) injected with dexamethasone-fibronectin microspheres from the same animal. The mean number of ganglion cells per linear mm of retina was not different between non-intervened and injected eyes. GCL: Ganglion cell layer; INL: Inner nuclear layer; ONL: Outer nuclear layer. Scale bars: (A) 1 mm, (B) 30 µm.

Article Snippet: Fibronectin quantification was performed by ELISA technique using a Fibronectin ELISA Kit (DuoSet® Human Fibronectin DY1918-05, R&D Systems, Minneapolis, MN, USA).

Techniques: Cell Analysis, Injection

Journal: Drug Delivery

Article Title: Mimicking chronic glaucoma over 6 months with a single intracameral injection of dexamethasone/fibronectin-loaded PLGA microspheres

doi: 10.1080/10717544.2022.2096712

Figure Lengend Snippet: Detection of microspheres in injected eyes. A. Paraffin sections stained with Hematoxylin/Eosin exhibited the presence of the microspheres integrated into the iridocorneal angle (arrowheads), a finding never observed in the control ones. B. The characteristic light diffraction produced by microspheres using Nomarski microscopy (arrowheads) confirmed their presence in the iridocorneal angle of injected eyes. C. The Fluorescent Stain BODIPY (green) also showed the presence of microspheres hampering the iridocorneal angle and the trabecular meshwork. Nuclei were counterstained with Hoechst (blue). LE: left eye (non-intervened); RE: Right eye (dexamethasone-fibronectin microsphere injected). CB: Ciliary body; I: Iris; C: cornea; TM: Transmission mode. Scale bars: 100 µm.

Article Snippet: Fibronectin quantification was performed by ELISA technique using a Fibronectin ELISA Kit (DuoSet® Human Fibronectin DY1918-05, R&D Systems, Minneapolis, MN, USA).

Techniques: Injection, Staining, Control, Produced, Microscopy, Transmission Assay